Thermo Fisher Scientific Alpha-1-Antitrypsin (SERPINA1) Monoclonal Antibody (AAT, 1378)

Applications

Western Blot (WB)

• Tested Dilution: 1–2 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1–2 µg/mL

Product Specifications

Product Specific Information

• Immunohistochemistry (PFA fixed): Incubate antibody for 30 min at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 min at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

Alpha-1-Antitrypsin (Alpha-1-AT) is synthesized in the liver and acts as an inhibitor of proteases such as trypsin, elastase, chymotrypsin, collagenase, leucocytic proteases, plasmin, and thrombin, which may be released during inflammatory reactions in the lung.
Deficiency of Alpha-1-AT can lead to chronic obstructive lung disease (emphysema) and, less frequently, hepatic cirrhosis in infants and respiratory distress in newborns.
Increased Alpha-1-AT levels occur as an acute phase response to tissue necrosis and inflammation. Serum levels are elevated in rheumatoid arthritis, bacterial infections, vasculitis, and carcinomatosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.