Thermo Fisher Scientific c-Fos Monoclonal Antibody (3G11), FITC

Applications

• Western Blot (WB): Tested dilution 1:1,000

Product Specifications

Product Specific Information

• A 1:1,000 dilution of MA5-45881 was sufficient for detection of c-Fos in 10 µg of serum-starved HeLa cells treated with PMA by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
• Detects 62 kDa band. Observed molecular weight of band is higher than predicted 41 kDa due to post-translational modifications.

Target Information

FBJ murine osteosarcoma viral oncogene homolog, commonly known as Cellular proto-oncogene Fos (c-Fos), belongs to the Fos transcription factor family and is encoded by the C-FOS gene located on chromosome 14.
c-Fos heterodimerizes with c-Jun to form the Activator Protein-1 (AP-1) transcription factor, which regulates transcription of genes involved in cellular signal transduction, proliferation, and differentiation.
It is phosphorylated by various stimuli including growth factors and insulin at multiple sites. The ERK MAPK kinase phosphorylates Threonine 232, regulating nuclear localization and transcriptional activity of c-Fos.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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