Thermo Fisher Scientific CD27 Monoclonal Antibody (O323), APC-eFluor 780, eBioscience

Thermo Fisher Scientific CD27 Monoclonal Antibody (O323), APC-eFluor 780, eBioscience

Applications

• Flow Cytometry (Flow): Tested at 5 µL (0.25 µg)/test
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Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 700
• APC
• Biotin
• Brilliant UV/Violet series
• eFluor 450
• FITC
• NovaFluor Blue/Red/Yellow series
• PE, PE-Cyanine5, PE-Cyanine7
• PerCP-eFluor 710
• Super Bright series
• Custom conjugation available

Product Specific Information

Description:
The O323 monoclonal antibody reacts with human CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by a subset of thymocytes and virtually all mature T cells, and is upregulated upon T-cell stimulation. CD27 binds to CD70, playing a crucial role in T cell-B cell interactions.

Applications Reported:
Suitable for flow cytometric analysis.

Applications Tested:
Pre-titrated and tested on normal human peripheral blood cells. Recommended use: 5 µL (0.25 µg)/test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test (empirical determination required).

Fluorophore Information:

• Emission: 780 nm
• Excitation: 633–647 nm (Red Laser)
• Tandem dye sensitive to photo-induced oxidation; protect from light.

Fixation:
Samples can be stored up to 3 days at 4°C in the dark using:

• IC Fixation Buffer (Product #00-8222)
• 1-step Fix/Lyse Solution (Product #00-5333)

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

CD27 is a 50 kDa member of the TNF receptor superfamily, including CD40 and CD30. It regulates lymphocyte proliferation and survival. CD27 is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral T cells, and certain B cells. Activation via TCR-CD3 increases CD27 expression and releases soluble CD27 (28–32 kDa), a marker for B cell malignancies.
CD27 interacts with proteins such as CD70 and SIVA, influencing apoptosis and immune regulation.
Associated diseases include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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