Thermo Fisher Scientific CD24 Monoclonal Antibody (M1/69), APC-eFluor 780, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 0.125 µg/test
  View 41 publications

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 532
• APC
• Biotin
• Brilliant UV 395 / 496 / 615 / 737 / 805
• Brilliant Violet 711 / 786
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 510 / 610-30S
• NovaFluor Red 685 / 710
• NovaFluor Yellow 570 / 590 / 660
• PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-Cyanine5.5 / PerCP-eFluor 710
• Super Bright 436 / 600 / 645
• Request custom conjugation

Product Specific Information

The M1/69 monoclonal antibody reacts with the mouse CD24 molecule, also known as Heat Stable Antigen (HSA). This 35–50 kDa molecule is anchored in the plasma membrane via phosphatidylinositol and is expressed by erythrocytes, thymocytes, peripheral lymphocytes, and myeloid lineage. CD24 is a variably glycosylated molecule resulting in heterogeneity of molecular mass on different cell lineages. Antibodies to CD24 show subtle differences in staining levels on lymphocyte populations. CD24 expression has been used to resolve stages of B lymphopoiesis in mouse bone marrow. P-selectin (CD62P) has been reported to bind to CD24.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤0.125 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize titration for best performance.

Fluorophore Information:
APC-eFluor 780 emits at 780 nm and is excited with the red laser (633 nm). Ensure instrument compatibility.

Light Sensitivity:
This tandem dye is sensitive to photo-induced oxidation. Protect from light.

Fixation:
Samples can be stored in:

• IC Fixation Buffer (Product #00-8222-49)
• 1-step Fix/Lyse Solution (Product #00-5333-54)
  for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency.

Excitation: 633–647 nm
Emission: 780 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD24 is a two-chain glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed throughout multiple stages of B-cell development, from pro-B cells in bone marrow to mature surface Ig-positive B cells. Plasma cell expression is low or absent. CD24 is also expressed in most B-lineage acute lymphoblastic leukemias, B-cell CCLs, and non-Hodgkin’s lymphomas. It may regulate B-cell proliferation, maturation, and autoimmunity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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