
Thermo Fisher Scientific MGMT Monoclonal Antibody (UMAB52), UltraMAB
MGMT 단백질을 인식하는 Thermo Fisher Scientific의 UMAB52 단클론 항체. Western blot, IHC, ICC/IF에 사용 가능하며, 인간 시료에 반응. 동결건조 형태로 제공되며, 재구성 후 약 1 mg/mL 농도로 사용. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | UMAB52 |
| Immunogen | Full length human recombinant protein of human MGMT produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
O6-Methylguanine-DNA Methyltransferase (MGMT) is a DNA repair protein that contributes to tumor cell resistance against chemotherapeutic alkylating agents. It also protects normal cells from genotoxic and carcinogenic effects of DNA alkylation. MGMT removes alkyl groups from the O6 position of guanine, and its expression varies by cell type, tissue, species, and tumor characteristics.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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