Thermo Fisher Scientific INSIG1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

-

Immunocytochemistry (ICC/IF)

1:100-1:1,000

-

Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to a region between residues 1-100 of human Insig1 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:INSIG1,uniProtId:O15503-1,ncbiNodeId:9606,antigenRange:1-100,antigenLength:277,antigenImageFileName:PA1-16988_INSIG1_O15503-1_Rabbit.svg,antigenImageFileNamePDP:PA1-16988_INSIG1_O15503-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.02 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 30% glycerol

Contains

0.1% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2126686

Product Specific Information

This antibody does not cross-react with Insig2.

Suggested positive control: HepG2 lysate, antigen standard for INSIG1 (transient overexpression lysate).

Target Information

Oxysterols regulate cholesterol homeostasis through liver X receptor (LXR) and sterol regulatory element-binding protein (SREBP) mediated signaling pathway. This gene is an insulin-induced gene. It encodes an endoplasmic reticulum (ER) membrane protein that plays a critical role in regulating cholesterol concentrations in cells. This protein binds to the sterol-sensing domains of SREBP cleavage-activating protein (SCAP) and HMG CoA reductase, and is essential for the sterol-mediated trafficking of the two proteins. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.