
Thermo Fisher Scientific Goat anti-Mouse IgG Fc Secondary Antibody, Biotin
염색 및 검출 실험용 염소 유래 항-마우스 IgG Fc 2차 항체로, Biotin 결합형입니다. Western blot, ICC/IF, Flow cytometry, ELISA 등 다양한 응용에 사용 가능하며, 높은 특이성과 민감도를 제공합니다. Affinity chromatography로 정제되어 신뢰성 높은 결과를 제공합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:20,000 |
| Flow Cytometry (Flow) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobin Fc region |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2534762 |
| Target | IgG Fc |
| Antibody Form | Whole Antibody |
Additional Formats
Product Specific Information
The sensitivity of each lot of antibody is confirmed using ELISA.
The specificity of each lot of antibody is confirmed by immunoelectrophoresis (IEP).
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in detection, sorting, or purification of their specified target.
Secondary antibodies offer increased versatility, enabling use with various detection systems (e.g., HRP, AP, fluorescence).
They can also provide greater sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically, these antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and may be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to produce highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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