
Thermo Fisher Scientific Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience
FITC로 결합된 Mouse IgG1 kappa 동형 대조 항체로, IHC, ICC/IF, Flow Cytometry에 사용 가능. 비특이적 결합 평가용으로 최적화됨. 488 nm 여기, 520 nm 방출 특성. 4°C 암소 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (IHC) | Assay-Dependent |
| Immunocytochemistry (ICC/IF) | Assay-Dependent |
| Flow Cytometry (Flow) | 5 µL (1 µg)/test |
| Control (Ctrl) | Assay-Dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Isotype Control |
| Clone | P3.6.2.8.1 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10598647 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 561, 647, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395–805 series
- Brilliant Violet 421–786 series
- eFluor 450, 506, 570, 615, 660
- Functional Grade
- Pacific Orange
- PE, PE-Cyanine5, 5.5, 7
- PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Custom conjugation available upon request
Product Specific Information
- Description: Monoclonal Mouse IgG1 κ immunoglobulin, used as an isotype control.
- Applications Reported: Suitable for immunocytochemistry, immunohistochemistry, and flow cytometry.
- Applications Tested: Pre-titrated; use 5 µL (1 µg) per test (100 µL final volume).
- Recommended cell count: 10⁵–10⁸ cells/test.
- Excitation: 488 nm
- Emission: 520 nm
- Laser: Blue Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
Mouse IgG1 is a subtype of immunoglobulin G produced by B cells in response to antigens. It is the most abundant IgG subtype in serum and plays a key role in the adaptive immune response.
It consists of two heavy and two light chains and is involved in:
- Opsonization
- Complement activation
- Antibody-dependent cell-mediated cytotoxicity
- Regulation of immune responses, including T cell activation and B cell inhibition
Mouse IgG1 is widely used in:
- ELISA
- Western blotting
- Immunohistochemistry
- Immunotherapy and vaccine development research
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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