Thermo Fisher Scientific ROBO1 Polyclonal Antibody
ROBO1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB 및 IHC(P) 실험에 적합합니다. Human에 반응하며 고순도 항원 친화 크로마토그래피로 정제되었습니다. 4°C 단기, -20°C 장기 보관이 권장됩니다.
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Thermo Fisher Scientific ROBO1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment containing a sequence corresponding to a region within amino acids 1219–1547 of ROBO1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2552174 |
Product Specific Information
- Recommended positive controls: HeLa, A375
- Predicted reactivity: Mouse (93%), Rat (92%), Xenopus laevis (84%), Bovine (95%)
- IHC notes: Requires antigen retrieval using heat-mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Specialized cells at the midline, which separates the left and right halves of the CNS, have roles in directing growth cone behavior. In vertebrate spinal cord, insect ventral nerve cord, and C. elegans, midline cells produce guidance cues such as nectins and slit, acting as attractants and repellents. Robo functions as a receptor for the repellent Slit and defines a subfamily of Ig superfamily proteins conserved from fruit flies to mammals. Non-crossing axons express high levels of Robo, while crossing axons express low levels before reaching the midline and high levels after crossing. Robo1 and Robo2 are human homologs of the Drosophila Roundabout protein. Robo1 is homologous to the C. elegans gene sax3; Robo2 to the zebrafish gene astray.
⚠ WARNING: This product can expose you to chemicals including mercury, known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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