Thermo Fisher Scientific POLI Polyclonal Antibody
Rabbit polyclonal antibody against human POLI protein. Validated for Western blot applications. Affinity purified with >95% purity. Supplied in PBS with glycerol for stability. For research use only.
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Applications
- Western Blot (WB): 1:500–1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant full-length Human POLI (UniProt ID: Q9UNA4-1; amino acids 1–740) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2720370 |
Product Specific Information
The antibody was affinity-purified from rabbit antiserum by epitope-specific affinity chromatography. The purity is greater than 95% as determined by SDS-PAGE.
Target Information
Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site and inserts the correct base with high fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, preferentially inserting guanosine. May play a role in hypermutation of immunoglobulin genes. Forms a Schiff base with 5′-deoxyribose phosphate at abasic sites, though may not have lyase activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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