Thermo Fisher Scientific Collagen 4 Polyclonal Antibody
Western blot, IHC, ICC/IF, Flow cytometry, ELISA 등 다양한 응용에 사용 가능. 사람, 마우스, 돼지, 랫트 반응성. Rabbit polyclonal IgG로 제작. KLH-conjugated synthetic peptide 면역원 사용. 단백질 정제 및 고순도 액상 형태로 제공.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:20–1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Pig, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide derived from human Collagen alpha-1 (IV) chain |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Collagen IV is a major structural component of the basement membrane in tissues such as kidney, lung, and skin. It forms a network-like structure that provides mechanical support and stability. Collagen IV consists of three alpha chains encoded by the COL4A1, COL4A2, and COL4A3 genes. Mutations in these genes are associated with genetic disorders such as Alport syndrome and Goodpasture syndrome, which primarily affect the kidneys and lungs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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