Thermo Fisher Scientific IFN gamma Monoclonal Antibody (4S.B3), PE-Cyanine7, eBioscience

Applications

• Flow Cytometry (Flow): Tested at ≤ 0.125 µg/test
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Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 660
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 615, 661, 737, 805
• Brilliant Violet 650
• eFluor 450, 506, 660
• FITC
• PE
• PE-eFluor 610
• PerCP-Cyanine5.5
• Request custom conjugation

Product Specific Information

Description:
The 4S.B3 monoclonal antibody reacts with interferon-gamma (IFN gamma). Human IFN gamma is a 17 kDa factor produced by activated T and NK cells and functions as an antiviral and antiparasitic cytokine. It works synergistically with TNF alpha to inhibit cell proliferation and modulates immune responses via high-affinity receptors on various cell types. Glycosylation does not affect its biological activity.

Applications Reported:
Reported for use in intracellular flow cytometric analysis.

Applications Tested:
Validated for intracellular flow cytometry at ≤ 0.125 µg/test (100 µL final volume). Cell number per test should be empirically determined (10⁵–10⁸ cells/test). Antibody titration is recommended for optimal assay performance.

Light Sensitivity:
This tandem dye is sensitive to photo-induced oxidation. Protect from light.

Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-57) for up to 3 days at 4°C in the dark with minimal signal loss.

Excitation / Emission / Laser:
488–561 nm excitation; 775 nm emission; compatible with Blue, Green, and Yellow-Green lasers.

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

IFN gamma (Interferon gamma, Type II interferon)
A macrophage activation factor and immune interferon produced mainly by T lymphocytes and NK cells in response to antigens and cytokines. It is a dimeric glycoprotein (~45 kDa) composed of two 146-amino-acid subunits. Biological activity is species-specific; human IFN gamma does not cross-react with mouse. Functions include antiviral and antiproliferative activity, induction of MHC I/II, macrophage activation, and enhanced immunoglobulin secretion by B cells. It acts through the JAK-STAT pathway via IFN gamma receptor I and II. Expression is upregulated by IL-2, FGF basic, EGF, and downregulated by vitamin D3 or DMN. Mutations are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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