Thermo Fisher Scientific IFN gamma Monoclonal Antibody (4S.B3), PE-Cyanine7, eBioscience

Thermo Fisher Scientific IFN gamma Monoclonal Antibody (4S.B3), PE-Cyanine7, eBioscience™

Applications and Tested Dilution

• Application: Flow Cytometry (Intracellular staining)
• Tested Dilution: 5 µL (0.125 µg)/test
• Publications: View 88 publications

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Product Specifications

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Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 660
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 496 / 615 / 661 / 737 / 805
• Brilliant Violet 650
• eFluor 450 / 506 / 660
• FITC
• PE / PE-eFluor 610
• PerCP-Cyanine5.5
• Request custom conjugation

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Product Specific Information

Description:
The 4S.B3 monoclonal antibody reacts with interferon-gamma (IFN-γ), a 17 kDa cytokine produced by activated T and NK cells with antiviral and antiparasitic activities. IFN-γ acts synergistically with cytokines such as TNF-α to inhibit cell proliferation and modulate immune responses across various cell types expressing IFN-γ receptors. Glycosylation does not affect its biological activity.

Applications Reported:
For intracellular flow cytometric analysis.

Applications Tested:
Pre-titrated and validated for intracellular flow cytometry. Use at 5 µL (0.125 µg) per test (100 µL final volume). Suitable for 10⁵–10⁸ cells/test.

Light Sensitivity:
Protect vial and stained samples from light due to photo-induced oxidation.

Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.

Excitation / Emission / Laser:
488–561 nm excitation; 775 nm emission; compatible with Blue, Green, and Yellow-Green lasers.

Filtration:
0.2 µm post-manufacturing filtered.

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Target Information

IFN gamma (Interferon gamma, Type II interferon):
A macrophage activation factor produced by T-lymphocytes and NK cells in response to antigens and cytokines. It is a dimeric glycoprotein (~45 kDa) composed of two 146 amino acid subunits. IFN-γ exhibits species-specific activity and does not cross-react between human and mouse. Functions include:

• Antiviral and antiproliferative activity
• Induction of MHC class I and II expression
• Macrophage activation
• Enhancement of immunoglobulin secretion by B lymphocytes

Activation occurs via IFN-γ receptor I/II and the JAK-STAT signaling pathway. Expression is upregulated by IL2, FGF basic, EGF, and downregulated by vitamin D3 or DMN. Mutations in the IFN-γ gene are associated with aplastic anemia.

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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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