Thermo Fisher Scientific RhoA Polyclonal Antibody
RhoA 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC/IF에 사용 가능. 인간 및 마우스 시료에 검증됨. 합성 펩타이드(aa 100-150)를 면역원으로 사용. 동결건조 형태로 제공되며, 장기 보관 시 -20°C 권장.
- 카탈로그번호
- OSR00266W
- 판매단위
- pk
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:300–1:2,000 | View 1 publication |
| Immunohistochemistry (IHC) | 1:300–1:2,000 | View 1 publication |
| Immunocytochemistry (ICC/IF) | – | View 1 publication |
Product Specifications
| Category | Specification |
|---|---|
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from aa region 100–150 of human RhoA conjugated to blue carrier protein was used as the antigen. |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not determined |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2253729 |
Product Specific Information
- Glycerol (1:1) may be added for additional stability.
- Reconstitute in 100 µL of sterile water.
- The peptide is homologous in many species including rat, mouse, chicken, dog, bovine, frog, zebrafish, and orangutan.
Target Information
RhoA regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. It is involved in a microtubule-dependent signal required for myosin contractile ring formation during cell cycle cytokinesis and plays an essential role in cleavage furrow formation.
RhoA is required for apical junction formation of keratinocyte cell-cell adhesion and serves as a target for the yopT cysteine peptidase from Yersinia pestis and Yersinia pseudotuberculosis.
RhoA stimulates PKN2 kinase activity and may activate PLCE1. It is activated by ARHGEF2, promoting the exchange of GDP for GTP, and is essential for SPATA13-mediated regulation of cell migration and adhesion assembly/disassembly.
The MEMO1–RHOA–DIAPH1 signaling pathway is important for ERBB2-dependent stabilization of microtubules at the cell cortex, controlling localization of APC and CLASP2 to the cell membrane via GSK3B regulation. Membrane-bound APC allows MACF1 localization, required for microtubule capture and stabilization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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