Thermo Fisher Scientific RhoA Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Glycerol (1:1) may be added for additional stability.
• Reconstitute in 100 µL of sterile water.
• The peptide is homologous in many species including rat, mouse, chicken, dog, bovine, frog, zebrafish, and orangutan.

Target Information

RhoA regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. It is involved in a microtubule-dependent signal required for myosin contractile ring formation during cell cycle cytokinesis and plays an essential role in cleavage furrow formation.
RhoA is required for apical junction formation of keratinocyte cell-cell adhesion and serves as a target for the yopT cysteine peptidase from Yersinia pestis and Yersinia pseudotuberculosis.
RhoA stimulates PKN2 kinase activity and may activate PLCE1. It is activated by ARHGEF2, promoting the exchange of GDP for GTP, and is essential for SPATA13-mediated regulation of cell migration and adhesion assembly/disassembly.
The MEMO1–RHOA–DIAPH1 signaling pathway is important for ERBB2-dependent stabilization of microtubules at the cell cortex, controlling localization of APC and CLASP2 to the cell membrane via GSK3B regulation. Membrane-bound APC allows MACF1 localization, required for microtubule capture and stabilization.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.