Thermo Fisher Scientific RPH3A Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:300–1:2,000

Immunohistochemistry (IHC)

• Tested Dilution: 1:300–1:2,000

Product Specifications

Product Specific Information

This antibody is predicted to react with human based on sequence homology.
Reconstitute with 100 µL of sterile water.

Target Information

The carboxy-terminal of Rabphilin-3A consists of two C2 domains (A and B), and the amino-terminal (residues 45–170) contains a cysteine-rich region with two zinc finger motifs. Rabphilin-3A belongs to a family of carboxy-terminal type (C-type) tandem C2 proteins, including synaptotagmins and Doc2.
Rabphilin is expressed in neuroendocrine cells and co-localizes with Rab3A on synaptic vesicles and chromaffin granules. It binds Rab3a/GTP/Mg²⁺ within amino-terminal residues 45–170 and interacts with calcium ions and phosphatidylinositol 4,5-bisphosphate-containing lipid vesicles within its C2 domains.
Rabphilin-3A acts as a positive regulator of calcium-dependent exocytosis, while Rab3a is a negative regulator. Although Rabphilin-3A associates with Rab3a, they influence exocytosis independently, likely mediated through interactions with an unknown factor recognizing the Rab3 binding domain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.