Thermo Fisher Scientific PRDX4 Monoclonal Antibody (OTI9A3), TrueMAB
PRDX4 단백질을 인식하는 마우스 단클론 항체로 Western blot, IHC, ICC/IF에 사용 가능. 인간 시료에 반응하며, 동결건조 형태로 제공. PBS 완충액(8% 트레할로스) 내 보관, -20°C에서 안정. 연구용으로 세포 내 산화환원 조절 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI9A3 |
| Immunogen | Full length human recombinant protein of human PRDX4 produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Peroxiredoxin (Prx) is a family of peroxidases associated with cell proliferation, differentiation, and apoptosis.
Mammalian Prx proteins (approximately 20–30 kDa) are categorized into typical 2-Cys, atypical 2-Cys, and 1-Cys Prx subgroups.
- Prx I, II, and VI: Cytosolic localization
- Prx III: Mitochondrial localization
- Prx IV: Endoplasmic reticulum and secretion
- Prx V: Present in peroxisome, mitochondria, and cytosol
Except for Prx VI (1-Cys subgroup), other 2-Cys Prx isotypes exhibit thioredoxin-dependent peroxidase activity using thioredoxin, thioredoxin reductase, and NADPH as reducing systems.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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