
Thermo Fisher Scientific Rabbit anti-Human IgE Recombinant Secondary Antibody, Biotin
Recombinant rabbit monoclonal secondary antibody specific for human IgE, conjugated with biotin for enhanced detection sensitivity. Suitable for ELISA applications. Provides high lot-to-lot consistency and animal-origin-free formulation. For research u...
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Thermo Fisher Scientific Rabbit anti-Human IgE Recombinant Secondary Antibody, Biotin
Applications
- ELISA (ELISA): 10–100 ng/well (Capture), 0.01–0.1 µg/mL (Detect)
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgE |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Secondary Antibody |
| Clone | RM122 |
| Immunogen | Human IgE |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2744975 |
| Target | IgE |
| Antibody Form | Whole Antibody |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal-origin-free formulations
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
Anti-Human secondary antibodies are affinity-purified with well-characterized specificity for human immunoglobulins. They are useful for detection, sorting, or purification of target molecules.
Secondary antibodies offer:
- Versatility for various detection systems (e.g., HRP, AP, fluorescence)
- Enhanced sensitivity via signal amplification
Typically generated by immunizing host animals with pooled human immunoglobulins, followed by purification and modification (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to ensure high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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