Thermo Fisher Scientific Bassoon Monoclonal Antibody (SAP7F407)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (IHC)

1:400

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

Assay-dependent

-

Immunoprecipitation (IP)

2.5 µg

-

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Mouse

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

SAP7F407

Immunogen

Recombinant, rat bassoon fragment (residues 738-1035) expressed as a fusion protein in E.coli. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Bassoon,uniProtId:O88778-1,ncbiNodeId:10114,antigenRange:738-1035,antigenLength:3938,antigenImageFileName:MA1-20689_Bassoon_O88778-1_House_mouse.svg,antigenImageFileNamePDP:MA1-20689_Bassoon_O88778-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with 0.1mM PMSF, 50% glycerol

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2066981

Product Specific Information

By Western blot, this antibody detects a ~400 kDa band represeneting the Bassoon protein. Additional bands between 97-400 kDa are also identified and are reported to be proteolytic degradation products of Bassoon.

Target Information

Bassoon is an approximately 420 kDa protein that is a localized at the presynaptic nerve terminals and is believed to play a role in the structural and functional organization of the synaptic vesicle cycle. Bassoon does not belong to any known protein families. It has been found in rat and mouse with sequence identity of the two proteins at 96%. The human BASSOON gene has recently been cloned and localized. Bassoon is predicted to contain two double-zinc fingers, three coiled-coil regions, and two polyglutamine domains. The polyglutamine domains in the C-terminus are of interest, since it is known that for some human proteins, such as Huntingtin, abnormal amplification of this region can cause late-onset neurodegeneration. Bassoon is concentrated at sites opposite to postsynaptic densities in synaptic terminals and in cultured neurons, it is found to colocalize with GABA (A) and glutamate (GluR1) receptors. Another presynaptic protein, Piccolo was found to colocalize with Bassoon in cultured hippocampal neurons. These observations suggested that they serve specific functions at synaptic junctions and may be involved in organization of the cytoskeleton at the site of neurotransmitter release.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.