Thermo Fisher Scientific EEF1B2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:2,500-1:10,000

-

ELISA (ELISA)

1:5,000-1:10,000

-

Immunoprecipitation (IP)

0.5-2 µL/mg of lysate

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant Human EF1B/EEF1B2 protein (Met1-Ile225) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:EEF1B2,uniProtId:P24534-1,ncbiNodeId:9606,antigenRange:1-225,antigenLength:225,antigenImageFileName:PA5-80572_EEF1B2_P24534-1_Rabbit.svg,antigenImageFileNamePDP:PA5-80572_EEF1B2_P24534-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A, Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2787871

Product Specific Information

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

This antibody has specificity for Human EF1B/EEF1B2.

Target Information

EF-1 (elongation factor-1) is a multi-protein complex that is responsible forthe delivery of aminoacyl-tRNAs to the ribosome. The EF-1 protein complexis composed of four subunits: EF-1 α, EF-1 β, EF-1 δ and EF-1 γ. EF-1 β is a225 amino acid member of the EF-1 β/EF-1 δ protein family and contains oneGST C-terminal domain. Both EF-1 β and EF-1 δ stimulate the exchange ofGDP bound to EF-1 α to GTP. Phosphorylation of EF-1 β affects the GDP/GTP exchange rate. Alternative splicing events of the gene that encodes EF-1 β result in three transcript variants that differ only in the 5` UTR.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.