Thermo Fisher Scientific F(ab`)2-Goat anti-Mouse IgG (H+L) Secondary Antibody, NovaFluor Yellow 590, eBioscience

Applications

Tested Dilution

Flow Cytometry (Flow)

Assay-dependent

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Goat / Ig

Class

Polyclonal

Type

Secondary Antibody

Conjugate

NovaFluor™ Yellow 590 NovaFluor™ Yellow 590 NovaFluor™ Yellow 590

View additional formats

• [Agarose](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Agarose/host/Goat/)
• [Alexa Fluor 488 ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Alexa Fluor 488/host/Goat/)
• [Alexa Fluor 488-10 nm colloidal gold](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Alexa Fluor 488-10 nm colloidal gold/host/Goat/)
• [Alexa Fluor 488-FluoroNanogold](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Alexa Fluor 488-FluoroNanogold/host/Goat/)
• [Alexa Fluor 555 ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Alexa Fluor 555/host/Goat/)
• [Alexa Fluor 594-FluoroNanogold](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Alexa Fluor 594-FluoroNanogold/host/Goat/)
• [Alexa Fluor 647 ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Alexa Fluor 647/host/Goat/)
• [Alexa Fluor 680 ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Alexa Fluor 680/host/Goat/)
• [Alexa Fluor 790 ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Alexa Fluor 790/host/Goat/)
• [Alexa Fluor Plus 405 ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Alexa Fluor Plus 405/host/Goat/)
• [Alexa Fluor Plus 488 ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Alexa Fluor Plus 488/host/Goat/)
• [Alexa Fluor Plus 647 ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Alexa Fluor Plus 647/host/Goat/)
• [AP](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/AP/host/Goat/)
• [APC ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/APC/host/Goat/)
• [bGal](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/bGal/host/Goat/)
• [Biotin ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Biotin/host/Goat/)
• [DyLight 350](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/DyLight 350/host/Goat/)
• [DyLight 405](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/DyLight 405/host/Goat/)
• [DyLight 488](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/DyLight 488/host/Goat/)
• [DyLight 550](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/DyLight 550/host/Goat/)
• [DyLight 594](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/DyLight 594/host/Goat/)
• [DyLight 633](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/DyLight 633/host/Goat/)
• [DyLight 650](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/DyLight 650/host/Goat/)
• [DyLight 680](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/DyLight 680/host/Goat/)
• [DyLight 800 4X PEG](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/DyLight 800 4X PEG/host/Goat/)
• [eFluor 570](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/eFluor 570/host/Goat/)
• [eFluor 660](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/eFluor 660/host/Goat/)
• [FITC](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/FITC/host/Goat/)
• [Functional Grade](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Functional Grade/host/Goat/)
• [HRP](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/HRP/host/Goat/)
• [NovaFluor Red 685](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/NovaFluor Red 685/host/Goat/)
• [NovaFluor Red 700](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/NovaFluor Red 700/host/Goat/)
• [NovaFluor Yellow 610](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/NovaFluor Yellow 610/host/Goat/)
• [NovaFluor Yellow 660](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/NovaFluor Yellow 660/host/Goat/)
• [PE ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/PE/host/Goat/)
• [PerCP-eFluor 710](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/PerCP-eFluor 710/host/Goat/)
• [Qdot 525](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Qdot 525/host/Goat/)
• [Qdot 565](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Qdot 565/host/Goat/)
• [Qdot 585](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Qdot 585/host/Goat/)
• [Qdot 605](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Qdot 605/host/Goat/)
• [Qdot 655](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Qdot 655/host/Goat/)
• [Qdot 705](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Qdot 705/host/Goat/)
• [Qdot 800](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Qdot 800/host/Goat/)
• [Rhodamine](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Rhodamine/host/Goat/)
• [TRITC](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/TRITC/host/Goat/)
• [WesternDot 625](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/WesternDot 625/host/Goat/)
• Request custom conjugation

Excitation/Emission Max

563/594 nm View spectra

Form

Liquid

Concentration

0.1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

RRID

AB_3099274 (AB_2921199)

Target

IgG

Antibody Form

F(ab`)2 Fragment

Product Specific Information

Description: The goat anti-mouse IgG polyclonal antibody reacts with the heavy and light chains of mouse IgG, with minimal cross reactivity to human, rat, bovine, goat, hamster, and rabbit serum proteins. Reactivity has been shown to IgG1, IgG2a, IgG2b, and IgG3, as well as some reactivity to IgM. The antibody is a F(ab`)2 fragment which contains the 2 Fab domains still linked through the hinge region of the immunoglobulin. Removal of the Fc region helps to reduce issues typically found with Fc-mediated binding.IThis polyclonal has been validated as a secondary reagent (its ability to recognize cells stained with a mouse IgG followed by this polyclonal antibody).

Each product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer .

Applications Reported: This polyclonal antibody has been reported for use in flow cytometric analysis.

Applications Tested: This polyclonal antibody has been tested by flow cytometric analysis of normal human peripheral blood cells stained first with a mouse anti-human antibody. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This polyclonal antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded sections that have been stained first with a mouse anti-human antibody. This can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

NovaFluor dyes are not compatible with DNA intercalating viability dyes. Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes.

This NovaFluor conjugate has been updated to ship with CellBlox Plus Blocking Buffer (Cat. No. (C001T06F01)). This buffer contains formulation improvements over CellBlox. CellBlox Plus Blocking Buffer is required for optimal staining with NovaFluor conjugates and should be used in all experiments where NovaFluor conjugates are used. Whenever possible, we recommend adding CellBlox Plus Blocking Buffer to antibody cocktails/master mixes prior to combining with cells. Add 5 µL per sample (regardless of the number of NovaFluors in your panel) to use the antibody cocktail as intended. For single-color controls, use 5 µL of CellBlox Blocking Buffer per 100 µL of cell sample containing 10^3 to 10^8 cells.

NovaFluor conjugates are based on Phiton™ technology utilizing novel nucleic acid dye structures that allow for engineered fluorescent signatures with consideration for spillover and spread impacts. Learn more

Excitation: 552 nm; Emission: 592 nm; Laser: 561 nm (Yellow) Laser

Target Information

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.