Thermo Fisher Scientific Factor XII Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 336-615 of human F12 (NP_0004962) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Factor XII,uniProtId:P00748-1,ncbiNodeId:9606,antigenRange:336-615,antigenLength:615,antigenImageFileName:PA5-116703_Factor_XII_P00748-1_Rabbit.svg,antigenImageFileNamePDP:PA5-116703_Factor_XII_P00748-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.43 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2901334

Product Specific Information

Positive Samples: HepG2, MCF7, Mouse liver

Immunogen sequence: QTPGALPAKR EQPPSLTRNG PLSCGQRLRK SLSSMTRVVG GLVALRGAHP YIAALYWGHS FCAGSLIAPC WVLTAAHCLQ DRPAPEDLTV VLGQERRNHS CEPCQTLAVR SYRLHEAFSP VSYQHDLALL RLQEDADGSC ALLSPYVQPV CLPSGAARPS ETTLCQVAGW GHQFEGAEEY ASFLQEAQVP FLSLERCSAP DVHGSSILPG MLCAGFLEGG TDACQGDSGG PLVCEDQAAE RRLTLQGIIS WGSGCGDRNK PGVYTDVAYY LAWIREHTVS

Target Information

This single chain zymogen is converted to a two-chain serine protease with an heavy chain (alpha-factor XIIa) and a light chain. The heavy chain contains two fibronectin-type domains, two epidermal growth factor (EGF)-like domains, a kringle domain and a proline-rich domain, whereas the light chain contains only a catalytic domain. On activation, further cleavages takes place in the heavy chain, resulting in the production of beta-factor XIIa light chain and the alpha-factor XIIa light chain becomes beta-factor XIIa heavy chain. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then to beta-factor XIIa. The active factor XIIa participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. It activates coagulation factors VII and XI. Defects in this gene do not cause any clinical symptoms and the sole effect is that whole-blood clotting time is prolonged.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.