Thermo Fisher Scientific Pepsin A Polyclonal Antibody
Thermo Fisher Scientific의 Pepsin A Polyclonal Antibody는 인간, 마우스, 랫트 반응성을 가지며 WB 및 IHC(P) 실험에 적합합니다. 42 kDa의 내인성 단백질을 검출하며, 항원 친화 크로마토그래피로 정제된 고순도 제품입니다. 연구용으로만 사용됩니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:50–1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 281–330 of human Pepsin A |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2553586 |
Product Specific Information
- Detects endogenous protein at a molecular weight of 42 kDa.
- Purity is >95% by SDS-PAGE.
Target Information
Pepsin consists of a single polypeptide chain and arises from its precursor, pepsinogen, by removal of a 41 amino acid segment from the N-terminus.
Pepsinogen is synthesized in the stomach lining, and hydrochloric acid is required to convert the inactive enzyme and maintain the optimum acidity (pH 1–3) for pepsin function.
Pepsin is particularly effective in cleaving peptide bonds involving aromatic amino acids and shows broad specificity.
Pepsin A, a member of the subfamily A1 within the pepsin family, is the predominant endopeptidase in the gastric juice of vertebrates and is inhibited by ovUS-1, a uterine serpin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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