Thermo Fisher Scientific CPI-17 Polyclonal Antibody
CPI-17 단백질을 인식하는 Rabbit Polyclonal 항체로, Human, Mouse, Rat 시료에 반응합니다. Western blot 및 IHC(Paraffin) 분석에 적합하며, 항원 친화 크로마토그래피로 정제되었습니다. 22 kDa 단백질 검출에 사용되며, 고순도와 안정적인 성능을 제공합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 2–50 of human CPI-17 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2553582 |
Product Specific Information
- Detects endogenous CPI-17 protein at a molecular weight of approximately 22 kDa.
- Purity >95% by SDS-PAGE.
Target Information
CPI-17 is a phosphorylation-dependent inhibitory protein for smooth muscle myosin phosphatase. It was originally identified as a PKC-potentiated inhibitory protein of protein phosphatase-1, dominantly expressed in smooth muscle. Phosphorylation at Threonine 38 by PKC or Rho-kinase enhances inhibitory potency toward myosin phosphatase. CPI-17 is also phosphorylated at Threonine 38 by protein kinase N and may be involved in calcium sensitization of smooth muscle contraction as a downstream effector of Rho and/or arachidonic acid. It is dually phosphorylated at Serine 12 and Threonine 38 by a MYPT-associated kinase (M110 kinase).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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