Thermo Fisher Scientific Pyruvate Kinase Polyclonal Antibody
Pyruvate Kinase에 특이적인 Thermo Fisher Scientific의 폴리클로날 항체로, Western blot, IHC, ELISA, IP에 사용 가능. Goat host, Rabbit reactant, Lyophilized 형태로 제공되며 4°C 또는 -20°C에서 안정적으로 보관 가능. 연구용으로만 사용.
- 카탈로그번호
- 100-1178
- 판매단위
- pk
카탈로그
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| ELISA | 1:50,000–1:200,000 |
| Immunoprecipitation (IP) | 1:5,000–1:20,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Rabbit |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Pyruvate Kinase [Rabbit Muscle] |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 40 mg/mL |
| Storage Buffer | 0.02M potassium phosphate/whole serum, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Anti-Pyruvate Kinase antibody immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Pyruvate Kinase [Rabbit Muscle].
This antibody reacts with all forms of pyruvate kinase (pan M-PK). Cross-reactivity against pyruvate kinase from other tissues and species may occur but has not been specifically determined.
Target Information
PKM2 is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. It interacts with thyroid hormone and may mediate metabolic effects induced by thyroid hormones.
PKM2 binds Opa protein, a bacterial outer membrane protein involved in gonococcal adherence and invasion of human cells, suggesting a role in bacterial pathogenesis.
Three alternatively spliced transcript variants encoding two distinct isoforms of PKM2 have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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