Thermo Fisher Scientific Pyruvate Kinase Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:20,000

-

Immunohistochemistry (IHC)

Assay-dependent

-

ELISA (ELISA)

1:50,000-1:200,000

-

Immunoprecipitation (IP)

1:5,000-1:20,000

-

Product Specifications

Species Reactivity

Rabbit

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Pyruvate Kinase [Rabbit Muscle] if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PKM2,uniProtId:P11974-1,ncbiNodeId:9986,antigenRange:1-531,antigenLength:531,antigenImageFileName:100-1178_PKM2_P11974-1_Goat.svg,antigenImageFileNamePDP:100-1178_PKM2_P11974-1_Goat_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

40 mg/mL

Storage buffer

0.02M potassium phosphate/whole serum, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Anti-PYRUVATE KINASE Antibody immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Pyruvate Kinase [Rabbit Muscle]. This product has been reported to react with all forms of pyruvate kinase (pan M-PK). Cross reactivity against Pyruvate Kinase from other tissues and species may occur but have not been specifically determined.

Target Information

PKM2 is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. PKM2 has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. PKM2 has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms of PKM2 have been reported.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.