Thermo Fisher Scientific CD244 Polyclonal Antibody
Thermo Fisher Scientific의 CD244 Polyclonal Antibody는 Mouse에 반응하는 Goat 유래 IgG 항체로, Western Blot, IHC, Flow Cytometry 등에 사용됩니다. 항원 친화 크로마토그래피로 정제되었으며, PBS와 trehalose buffer로 보관됩니다. 연구용으로만 사용됩니다.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1 µg/mL | - |
| Immunohistochemistry (IHC) | 5–15 µg/mL | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 1 publication |
| Flow Cytometry (Flow) | 2.5 µg per million cells | - |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant mouse 2B4/CD244/SLAMF4 His17–Asn221 |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No Preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2608062 |
Product Specific Information
- In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) 2B4 is observed and less than 1% cross-reactivity with rhSLAM is observed.
- Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
CD244 (2B4, SLAMF4) is a cell surface receptor expressed on natural killer (NK) cells and some T cells that mediate non-major histocompatibility complex (MHC) restricted killing.
Interaction between NK cells and target cells via CD244 modulates NK-cell cytolytic activity.
CD244 interacts with SLAMF2, activating both SLAMF4- and SLAMF2-expressing cells.
Patients with systemic lupus erythematosus exhibit reduced SLAMF4 levels on NK cells and monocytes, suggesting a role in autoimmune pathology.
Diseases associated with CD244 dysfunction include rheumatoid arthritis and lymphoproliferative syndrome.
Alternatively spliced transcript variants encoding different isoforms of CD244 have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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