Thermo Fisher Scientific CARD8 Polyclonal Antibody
Human CARD8 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC, ELISA 등 다양한 응용에 사용 가능. KLH-conjugated synthetic peptide(aa 221-320)를 면역원으로 사용. Protein A 정제, 1 mg/mL 농도, -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide derived from human CARD8 (amino acids 221–320) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Apoptosis is related to many diseases and developmental processes. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD-containing proteins include caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, BCL-10, and ASC.
CARD8 is a newly identified CARD-containing protein that interacts with DRAL, a p53-responsive protein involved in apoptosis induction, as well as caspase-1 and related proteins ICEBERG and pseudo-ICE. Although reports differ on whether CARD8 acts as a pro- or anti-apoptotic protein, it is suggested to function as an adaptor molecule regulating caspase-1 and NF-κB activation.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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