Takara BciT130I (EcoRII, MvaI) restriction enzyme
BciT130I(EcoRII, MvaI)는 Bacillus circulans T130 유래 제한효소로 DNA 메틸화에 영향을 받지 않으며 37°C에서 반응합니다. 고농도 글리세롤이나 DMSO 존재 시 비특이적 절단 가능성이 있습니다. 완전 불활성화를 위해 에탄올 침전 처리가 필요합니다. 고품질 QC 테스트를 거친 연구용 효소입니다.
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Takara · Takara BciT130I (EcoRII, MvaI) restriction enzyme
BciT130I (EcoRII, MvaI) Restriction Site
Recognition Sequence:
CC | (A/T)GG
GG(T/A) | CCSupplied Buffer: K
Effect of DNA Methylation: Enzyme activity is not affected by dcm methylase
Reaction Temperature: 37°C
Product Specifications
| 항목 | 내용 |
|---|---|
| Source | Bacillus circulans T130 |
| Concentration | 10 units/µl |
| Substrate for Unit Definition | Lambda DNA |
| Inactivation Treatment | Heat treatment (70°C, 15 min) is not sufficient; requires ethanol precipitation or phenol + ethanol precipitation |
| Ligation-Recutting Test | Ligation is inefficient due to fragment end structure; overnight ligation using DNA Ligation Kit Ver. 2.1 recommended |
| Star Activity | Unrelated sites may be cut in presence of high glycerol, DMSO, or low ionic strength |
| Quality Testing | Each lot tested for overdigestion, genome DNA analysis, ligation-recutting, and pKF3 cloning |
Additional Product Information
Please refer to the product’s Certificate of Analysis for storage conditions, product components, and technical specifications.
Kit Components Lists and Certificates of Analysis are available under the Documents tab.
Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566
Asia Pacific: +1.650.919.7300
Europe: +33.(0)1.3904.6880
Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
© 2023 Takara Bio Inc. All Rights Reserved.
All trademarks are the property of Takara Bio Inc. or its affiliates.
Additional product and intellectual property information available at takarabio.com
Takara 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.