Takara BciT130I (EcoRII, MvaI) restriction enzyme

BciT130I (EcoRII, MvaI) restriction site:

CC | (A/T)GG
GG(T/A) | CC

Supplied buffer: K

Effect of DNA methylation: enzyme activity is not affected by dcm methylase

Reaction temperature: 37°C

More Information

• Source: Bacillus circulans T130
• Concentration: 10 units/µl
• Substrate for unit definition: lambda DNA
• Inactivation treatment: Heat treatment (70°C, 15 min) is not sufficient to inactivate this enzyme completely. It is necessary to inactivate this enzyme by ethanol precipitation treatment (or phenol treatment followed by ethanol precipitation treatment)
• Ligation-recutting test: After digestion with this enzyme, the ligation is inefficient under the influence of the structure of BciT130I fragment ends. For ligation, the overnight ligation using DNA Ligation Kit Ver. 2.1 is recommended.
• Star activity: unrelated sites may be cut in the presence of high concentrations of glycerol, DMSO, or low ionic strength
• Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2023 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.