Takara BamHI restriction enzyme
BamHI 제한효소는 E. coli 유래 효소로, CG·dam·dcm 메틸화에 영향을 받지 않습니다. 30°C에서 최적 활성를 가지며 37°C에서도 동일한 활성을 보입니다. 고농도에서도 높은 품질을 유지하며, 각 로트는 엄격한 품질 검증을 거칩니다.
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BamHI Restriction Enzyme
Recognition Site
G | GATC C
C CTAG | GReaction Conditions
- Supplied Buffer: K
- Effect of DNA Methylation: Enzyme activity is not affected by CG methylase, dam methylase, or dcm methylase
- Reaction Temperature: 30°C
Specifications
| 항목 | 내용 |
|---|---|
| Source | Escherichia coli carrying the plasmid encoding BamHI gene |
| Concentration | 8–20 units/μl (High concentration: 30–60 units/μl) |
| Substrate for Unit Definition | Lambda DNA |
| Star Activity | Unrelated sites may be cut in the presence of high glycerol, Mn²⁺, or DMSO, and at low ionic strength |
| Activity Notice | Equivalent activity at 37°C and 30°C; enzyme stability is lower at 37°C |
| Quality Testing | Each lot tested for overdigestion, genome DNA analysis, ligation-recutting, and pKF3 cloning |
Additional Product Information
Please refer to the product’s Certificate of Analysis for details on storage conditions, product components, and technical specifications.
Kit Components List and Certificates of Analysis are available under the Documents tab.
Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566
Asia Pacific: +1.650.919.7300
Europe: +33.(0)1.3904.6880
Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
© 2023 Takara Bio Inc. All Rights Reserved.
All trademarks are the property of Takara Bio Inc. or its affiliates.
Additional product and intellectual property information available at takarabio.com.
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