Takara BamHI restriction enzyme

BamHI restriction site:

G | GATC C
C CTAG | G

Supplied buffer: K

Effect of DNA methylation: enzyme activity is not affected by CG methylase, dam methylase, or dcm methylase

Reaction temperature: 30°C

More Information

• Source: Escherichia coli carrying the plasmid encoding _Bam_HI gene
• Concentration: 8–20 units/μl [High concentration: 30–60 units/μl]
• Substrate for unit definition: lambda DNA
• Star activity: unrelated site may often be cut in the presence of high concentration of glycerol, Mn2+, or DMSO, and at low ionic strength
• Notice: this enzyme shows equivalent activity at 37°C and to 30°C, but the stability of the enzyme is lower in reaction mixtures at 37°C than that at 30°C
• Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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