Takara BcnI (CauII) restriction enzyme

BcnI (CauII) restriction site:

CC | (C/G)GG
GG(G/C) | CC

Supplied buffer: basal

Reaction temperature: 37°C

More Information

• Source: Bacillus centrosporus RFL 1
• Concentration: 10 units/µl
• Substrate for unit definition: lambda DNA
• Ligation-recutting test: After digestion with this enzyme, the ligation is inefficient under the influence of the structure of BcnI fragments ends (Janulaitis, A.A., personal information). For circularized ligation, overnight ligation using TaKaRa DNA Ligation Kit Ver. 2.1 or Mighty Mix is recommended.
• Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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