
Thermo Fisher Scientific beta-2-Microglobulin Polyclonal Antibody
Human beta-2-Microglobulin 단백질을 항원으로 제작된 Rabbit Polyclonal Antibody. 다양한 응용 분야(WB, IHC, ICC, ELISA, IP)에 사용 가능. 고순도 IgG 분획으로 제조되어 높은 특이성과 안정성 제공. 연구용으로만 사용.
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Thermo Fisher Scientific beta-2-Microglobulin Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:200 |
| ELISA | 1:2,000–1:10,000 |
| Immunoprecipitation (IP) | 1:200–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Human beta-2-Microglobulin protein isolated from urine |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | 0.02M potassium phosphate (pH 7.2), 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C and avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid freeze/thaw cycles. Centrifuge if not clear after standing at room temperature. Stable for several weeks at 4°C as undiluted liquid. Dilute only prior to immediate use.
This antibody is purified from monospecific antiserum by delipidation, salt fractionation, and ion-exchange chromatography, followed by dialysis. Immunoelectrophoresis shows a single precipitin arc against anti-Peroxidase and anti-Rabbit Serum, as well as purified and partially purified human beta-2-Microglobulin. Cross-reactivity with other sources is unknown.
Target Information
Beta-2-microglobulin (B2M) is a 12–14 kDa subunit of MHC class I molecules. It is associated with HLA Class I antigens expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial and epithelial cells, but absent on erythrocytes. Found both membrane-bound and free in plasma, B2M has sequence homology to immunoglobulin constant regions and a beta-pleated sheet structure. It plays roles in antigen presentation, TCR signaling, and CD8+ cell signaling. The B2M gene is located on chromosome 15; mutations can cause hypercatabolic hypoproteinemia. Beta-2-microglobulin levels serve as renal function markers and as prognostic indicators for HIV infection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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