Thermo Fisher Scientific PCNA Polyclonal Antibody
PCNA 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC/IF 등 다양한 응용에 사용 가능. 세포 증식 및 DNA 복제 관련 연구에 적합하며, 핵 내 PCNA 발현 검출에 유용. 메탄올 고정 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | View 1 publication |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | - |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant human PCNA protein (amino acids 1–261) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10984781 |
Product Specific Information
Methanol fixation is recommended for IF applications. Paraformaldehyde fixation for IF will result in mostly nuclear staining with some cytoplasmic staining.
Target Information
PCNA (Proliferating Cell Nuclear Antigen) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. It binds to CDK inhibitor p21, endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT).
PCNA serves as a marker of proliferative cells and is useful for assessing tumor proliferation status. It is expressed in the nucleus, acts as a cofactor of DNA polymerase delta, and forms a homotrimer to enhance leading strand synthesis during DNA replication.
Upon DNA damage, PCNA becomes ubiquitinated and participates in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been identified, and pseudogenes exist on chromosome 4 and the X chromosome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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