Thermo Fisher Scientific DIO3 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5 µg/mL

-

Immunohistochemistry (IHC)

Assay-Dependent

-

Immunocytochemistry (ICC/IF)

1:10-1:500

-

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide made to an internal portion of the rat protein (within residues 1-100). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:DIO3,uniProtId:P49897-1,ncbiNodeId:10114,antigenRange:1-100,antigenLength:304,antigenImageFileName:PA5-22886_DIO3_P49897-1_Rabbit.svg,antigenImageFileNamePDP:PA5-22886_DIO3_P49897-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_11156216

Product Specific Information

The target sequence has 93% sequence homology with hamster.

Suggested positive control: rat placenta tissue lysate.

Target Information

DIO3 belongs to the iodothyronine deiodinase family. It catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3,3,5-triiodothyronine (T3) to inactive metabolites, 3,3,5-triiodothyronine (RT3) and 3,3-diiodothyronine (T2), respectively. This enzyme is highly expressed in the pregnant uterus, placenta, fetal and neonatal tissues, suggesting that it plays an essential role in the regulation of thyroid hormone inactivation during embryological development. This protein contains a selenocysteine (Sec) residue, which is essential for efficient enzyme activity. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3` UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.