Thermo Fisher Scientific DIO3 Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• The target sequence has 93% sequence homology with hamster.
• Suggested positive control: rat placenta tissue lysate.

Target Information

DIO3 belongs to the iodothyronine deiodinase family. It catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3,3′,5-triiodothyronine (T3) to inactive metabolites, 3,3′,5′-triiodothyronine (rT3) and 3,3′-diiodothyronine (T2), respectively.
This enzyme is highly expressed in the pregnant uterus, placenta, fetal, and neonatal tissues, suggesting that it plays an essential role in the regulation of thyroid hormone inactivation during embryological development.
This protein contains a selenocysteine (Sec) residue, which is essential for efficient enzyme activity. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3′ UTR of Sec-containing genes has a common stem-loop structure, the SECIS (selenocysteine insertion sequence), necessary for recognition of UGA as a Sec codon rather than as a stop signal.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.