Thermo Fisher Scientific Aquaporin 2 Polyclonal Antibody
Aquaporin 2 단백질을 검출하기 위한 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, Western blot, IHC, ICC/IF 등 다양한 응용에 적합합니다. 인간, 마우스, 랫트 반응성을 가지며, 항원 친화 크로마토그래피로 정제된 고품질 항체입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | – |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:200 | – |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | – |
| Immunocytochemistry (ICC/IF) | 1 µg/mL | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide made to a C-terminus portion of the rat protein (within residues 200–300) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11154667 |
Product Specific Information
In Western blot, bands can be seen at approximately 28 kDa for the non-glycosylated form and 37 kDa for the glycosylated form.
Suggested positive control: rat kidney, renal inner medulla.
Target Information
Aquaporin 2 (AQP2) is a hormonally regulated water channel located in the renal collecting duct. Mutations in the AQP2 gene cause hereditary nephrogenic diabetes insipidus in humans. A vasopressin-induced cAMP increase results in phosphorylation of AQP2 at serine-256 and its translocation from intracellular vesicles to the apical membrane of principal cells.
Recently, serine-261 has been identified as a novel phosphorylation site on AQP2, and levels of phosphorylated S261 decrease with vasopressin treatment, suggesting its involvement in vasopressin-dependent AQP2 trafficking.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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