Thermo Fisher Scientific L-Plastin Recombinant Rabbit Monoclonal Antibody (3T0M1)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

3T0M1

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 528-627 of human L-Plastin/LCP1 (P13796). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:L-Plastin,uniProtId:P13796-1,ncbiNodeId:9606,antigenRange:528-627,antigenLength:627,antigenImageFileName:MA5-42856_L-Plastin_P13796-1_Rabbit.svg,antigenImageFileNamePDP:MA5-42856_L-Plastin_P13796-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.24 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2911997

Product Specific Information

Positive test controls include: Raji.

Immunogen sequence: TLREAKKSSS ISSFKDPKIS TSLPVLDLID AIQPGSINYD LLKTENLNDD EKLNNAKYAI SMARKIGARV YALPEDLVEV NPKMVMTVFA CLMGKGMKRV

Target Information

L-plastin is a leukocyte specific actin bundling protein from fimbrin family of proteins. It has two actin-binding domains and two EF hand type calcium-binding domains. It gets phosphorylated on the serine residue in its headpiece region in response to a variety of inflammatory stimuli. It is hypothesized that L-plastin may play a role in regulating integrin mediated adhesion in leukocytes. L-plastin is expressed in majority of human cancer cell lines derived from solid tumors. Its expression is positively upregulated by testosterones in androgen receptor positive prostrate and breast cancer cells through three androgen receptor binding elements located in the upstream region of the gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.