Thermo Fisher Scientific 14-3-3 beta Monoclonal Antibody (5B5G10)
14-3-3 beta 단백질을 검출하는 Mouse monoclonal antibody. WB, IHC, ICC, Flow, ELISA 등 다양한 응용 가능. Human, Mouse, Rat 반응성. 고순도 Protein G 정제, 1 mg/mL 농도. 연구용으로만 사용.
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Thermo Fisher Scientific 14-3-3 beta Monoclonal Antibody (5B5G10)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5B5G10 |
| Immunogen | Purified recombinant fragment of human YWHAB (aa: 1–246) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2898575 |
Product Specific Information
This antibody has been tested in indirect ELISA.
Target Information
14-3-3 is a family of acidic, soluble proteins highly conserved across eukaryotic species. Seven isoforms (beta, gamma, epsilon, eta, zeta, sigma, and tau/theta) are identified in mammals, forming homo- and heterodimeric cup-shaped structures.
14-3-3 interacts with over 100 binding partners, regulating key proteins involved in signal transduction, cell cycle, transcriptional control, cell proliferation, apoptosis, and ion channel physiology.
Most 14-3-3 interactions require phosphorylation of serine or threonine residues in target sequences. This protein is abundantly expressed in the brain and detected in cerebrospinal fluid of patients with various neurological disorders.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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