Thermo Fisher Scientific CTCF Monoclonal Antibody (CL0307)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:500

-

Immunocytochemistry (ICC/IF)

2-10 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

CL0307

Immunogen

Recombinant Protein corresonding to Human CTCF if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CTCF,uniProtId:P49711-1,ncbiNodeId:9606,antigenRange:42-189,antigenLength:727,antigenImageFileName:MA5-34975_CTCF_P49711-1_House_mouse.svg,antigenImageFileNamePDP:MA5-34975_CTCF_P49711-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2848880

Product Specific Information

Immunogen sequence: QNQTDGGEVV QDVNSSVQMV MMEQLDPTLL QMKTEVMEGT VAPEAEAAVD DTQIITLQVV NMEEQPINIG ELQLVQVPVP VTVPVATTSV EELQGAYENE VSKEGLAESE PMICHTLPLP EGFQVVKVGA NGEVETLEQG ELPPQEDP

Target Information

Transcriptional factor CTCF is a ubiquitously expressed 11-zinc finger domain containing, DNA-binding nuclear phosphoprotein that is involved in multiple aspects of normal gene regulation including transcriptional repression and activation, gene silencing, chromatin insulation and regulation of imprinted sites. CTCF uses different combinations of ZF domains and interacts with the CCCTC motif on the DNA. It can bind to HAT- and HDAC-containing complexes thereby influencing transcriptional activation or repression respectively. It is a multifunctional protein involved in the transactivation of APPB gene, silencing of c-myc gene, and insulation of human beta-globin and DMI myotonic dystrophy locus and imprinting control of IGF2 and H19 regions. CTCF has also been shown to regulate the transcription of Pax6 and IRAK2 promoter thereby opening a therapeutic avenue. Mutations in this gene have been associated with invasive breast cancers, prostate cancers and Wilms` tumors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.