Thermo Fisher Scientific Phospho-JNK1/JNK2 (Thr183, Tyr185) Polyclonal Antibody
인간, 생쥐, 랫드의 JNK1/JNK2 인산화 형태(Thr183, Tyr185)를 특이적으로 인식하는 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF 등 다양한 응용에 적합. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 재현성 제공.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 52 publications |
| Immunohistochemistry (IHC) | Assay-dependent | View 4 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:250 | View 2 publications |
| Immunoprecipitation (IP) | - | View 1 publication |
| Miscellaneous PubMed (Misc) | - | View 3 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Insect, Mouse, Non-human primate, Pig, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Chemically synthesized phosphopeptide derived from human JNK1&2 containing Thr183 and Tyr185. Conserved among mouse, rat, chicken, nematode, fruit fly, and JNK3. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Dulbecco’s PBS, pH 7.3, with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533720 |
Product Specific Information
This antibody is reactive to human and rat JNK1&2. Other species have not been tested, and JNK3 (found primarily in neuronal cell lines) has not been detected. The antibody has been negatively preadsorbed using a non-phosphopeptide to remove reactivity with non-phosphorylated JNK1&2. The final product is purified by affinity chromatography using a peptide phosphorylated at Thr183 and Tyr185.
Positive controls: HEK 293 ± UV irradiation; PC12 cells ± sorbitol.
Target Information
C-Jun N-terminal Kinase (JNK), also known as Mitogen-activated protein kinase (MAPK8), is part of the MAPK superfamily of stress-activated protein kinases. These Serine-threonine kinases mediate signal transduction from the cell surface to the nucleus and are involved in proliferation, differentiation, transcription regulation, and development. JNK pathways are activated by stress and inflammatory signals. JNK exists as ten isoforms due to differential mRNA splicing, with JNK1 and JNK2 being predominant.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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