Thermo Fisher Scientific TNP2 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

Immunocytochemistry (ICC/IF)

• Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

The antibody was affinity-purified from rabbit antiserum by affinity chromatography using an epitope-specific immunogen. The purity is greater than 95% as determined by SDS-PAGE.

Target Information

During mammalian spermiogenesis, histones are transiently replaced by several low molecular weight proteins called transition proteins (TNPs). Transition proteins facilitate chromatin transformation from the nucleosome structure to the nucleoprotamine structure during spermatid differentiation.

Transition protein-2 (TNP2), also known as TP2, maps to human chromosome 16p13.13 and encodes a highly basic nuclear protein. TNP2 is a spermatid-specific product of the haploid genome that replaces histones and is later replaced by protamines in mature sperm. TNP2 is not essential for sperm nucleus shaping, histone displacement, initiation of chromatin condensation, or fertility, but it is necessary for the normal processing of protamine 2 and the completion of chromatin condensation. If TNP1 is absent, TNP2 may partially compensate, though this can lead to abnormal chromatin condensation and reduced fertility.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.