Thermo Fisher Scientific ChAT Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

-

Immunohistochemistry (IHC)

-

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:1,000

-

Immunocytochemistry (ICC/IF)

1:100-1:1,000

-

Functional Assay (Functional)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 102 and 351 of Human CHAT if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ChAT,uniProtId:P28329-1,ncbiNodeId:9606,antigenRange:102-351,antigenLength:748,antigenImageFileName:PA5-29653_ChAT_P28329-1_Rabbit.svg,antigenImageFileNamePDP:PA5-29653_ChAT_P28329-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.15 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 1% BSA, 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2547128

Product Specific Information

Recommended positive controls: IMR32, mouse brain, rat brain, DDDDK-tagged CHAT-transfected 293T.

Predicted reactivity: Mouse (93%), Rat (92%), Pig (94%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

ChAT catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.