Thermo Fisher Scientific CD14 Polyclonal Antibody
Thermo Fisher Scientific의 CD14 Polyclonal Antibody는 마우스 및 랫트에 반응하며, WB·IHC·Flow Cytometry 등 다양한 응용에 적합합니다. 항원 친화 크로마토그래피로 정제된 비결합형 항체로, E. coli 유래 CD14 단백질을 면역원으로 사용합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.5 µg/mL | – |
| Immunohistochemistry (IHC) | – | 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 0.5–1 µg/mL | – |
| Flow Cytometry (Flow) | 1–3 µg/1×10^6 cells | 1 publication |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli-derived mouse CD14 recombinant protein (Position: S16–A260) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 500 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 4 mg trehalose |
| Contains | 0.05 mg sodium azide |
| Storage Conditions | −20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2746073 |
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
CD14 is a 55 kDa GPI-anchored glycoprotein expressed on the surface of mature monocytes, macrophages, and neutrophils. It functions as a multifunctional lipopolysaccharide receptor and can exist in both membrane-bound and soluble forms. CD14 interacts with lipopolysaccharide-binding protein (LBP) to bind lipopolysaccharides and plays a role in immune response modulation. Soluble CD14 (sCD14) discriminates structural differences between lipopolysaccharides and contributes to neutralization of foreign lipopolysaccharides. Additionally, CD14 binds apoptotic cells and influences allergic, inflammatory, and infectious processes. Alternative splicing produces multiple transcript variants encoding the same isoform. CD14 dysfunction is associated with mycobacterium chelonae infection and Croup.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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