Thermo Fisher Scientific CD4 Monoclonal Antibody (GK1.5), NovaFluor Blue 530, eBioscience

Thermo Fisher Scientific CD4 Monoclonal Antibody (GK1.5), NovaFluor Blue 530, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.2 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV/Violet/Yellow series
• eFluor 450, 506, 660
• FITC, PE, PE-Cyanine5, PE-Cyanine7
• Super Bright series
• NovaFluor Blue, Red, UV, Violet, Yellow variants
• Functional Grade
• Custom Conjugation Available

Product Specific Information

The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by most thymocytes, subsets of mature T cells, and dendritic cells. CD4 binds MHC class II on antigen-presenting cells and plays a critical role in T cell development and function. In T cells, CD4 associates with protein tyrosine kinase p56^lck through its cytoplasmic tail. Binding of GK1.5 is blocked by RM4-5.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Mouse splenocytes, ≤0.2 µg/test (100 µL final volume, 10⁵–10⁸ cells/test)

Usage Note:

• Carefully titrate for optimal performance.
• NovaFluor dyes are incompatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
  • Add 5 µL per sample before combining with cells.
  • For single-color controls: 5 µL per 100 µL cell sample (10³–10⁸ cells).

NovaFluor Technology:
Based on Phiton™ nucleic acid dye structures engineered for optimized fluorescent signatures with minimal spillover and spread.
Excitation: 509 nm; Emission: 530 nm; Laser: 488 nm (Blue)

Target Information

CD4 is a co-receptor involved in the recognition of MHC class II molecules and HIV binding. It is mainly expressed in T helper cells but also in monocytes, macrophages, and dendritic cells. Tissue expression includes thymus, lymph nodes, tonsils, spleen, and certain regions of brain and gut. CD4 participates in early T-cell activation via association with the T-cell receptor complex and Lck kinase, and may mediate neuronal damage in immune-related CNS diseases. Multiple alternatively spliced transcripts have been identified.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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