Thermo Fisher Scientific CD183 (CXCR3) Monoclonal Antibody (CXCR3-173), FITC, eBioscience

Thermo Fisher Scientific CD183 (CXCR3) Monoclonal Antibody (CXCR3-173), FITC, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats

• APC
• Biotin
• Brilliant Violet 421
• Brilliant Violet 480
• Functional Grade
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436
• Super Bright 600
• Request custom conjugation available

Product Specific Information

The monoclonal antibody CXCR3-173 recognizes mouse CD183 (CXCR3), a seven-transmembrane G-protein-linked chemokine receptor binding CXCL9 (Mig), CXCL10 (IP-10), and CXCL11 (ITAC).
CD183 plays a role in CD4 T cell responses to grafts, and CXCR3 knockout mice show reduced allograft rejection. Expression is found on NK cells, subsets of T lymphocytes and Tregs, and preferentially on Th1-polarized cells.

CXCR3-173 antibody affects chemotaxis in response to ligand; ligand presence eliminates staining. In vivo, antibody addition delays cardiac and pancreatic islet allograft rejection.

Applications Reported: Flow cytometric analysis.
Applications Tested: Flow cytometric analysis of mouse splenocytes, ≤0.25 µg/test (100 µL final volume, 10⁵–10⁸ cells/test).
Careful titration recommended for optimal performance.

Excitation: 488 nm
Emission: 520 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This gene encodes a G protein-coupled receptor selective for chemokines IP10, Mig, and I-TAC. These belong to the CXC chemokine family, characterized by conserved cysteine residues.
Binding induces cellular responses related to leukocyte traffic, integrin activation, cytoskeletal changes, and chemotaxis. Inhibition by Bordetella pertussis toxin suggests Gi-class G protein coupling.
Signal transduction may involve enzymes similar to other chemokine receptors. Chemokine-induced desensitization leads to rapid, short cellular responses, restored by receptor recycling.
Expression is high in effector/memory T cells and inflamed tissues. IP10, Mig, and I-TAC are produced locally in inflammation, suggesting roles in inflammatory cell recruitment.
This receptor is a target for small-molecule antagonists for inflammatory disease treatment. Multiple transcript variants exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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