Thermo Fisher Scientific CD199 (CCR9) Monoclonal Antibody (eBioCW-1.2 (CW-1.2)), FITC, eBioscience

Thermo Fisher Scientific CD199 (CCR9) Monoclonal Antibody (eBioCW-1.2 (CW-1.2)), FITC, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.25 µg/test
Publications: View 9 publications

Product Specifications

Additional Formats:

• APC
• eFluor 450
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The eBioCW-1.2 monoclonal antibody reacts with mouse CCR9 (CD199), which is the receptor for thymus-expressed chemokine (TECK). CCR9 is a member of the G protein coupled receptor (GPCR) supergene family, and is involved in trafficking of T cell progenitors within the thymus.

CCR9 expression during thymocyte development commences at the double-negative (DN) 3 stage (CD4-CD8-CD25+CD44-), peaks in the double-positive (DP) stage (CD4+CD8+CD25-CD44-), and is down-regulated in committed CD4+ or CD8+ single-positive (SP) thymocytes. CCR9-deficient mice show a mild impairment in thymocyte development.

In the periphery, CCR9 is thought to be expressed on naive CD8+ T cells, but not on naive CD4+ T cells.

Applications Reported: This eBioCW-1.2 (CW-1.2) antibody has been reported for use in flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of mouse thymocytes and/or splenocytes. Can be used at ≤0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically (10⁵–10⁸ cells/test). It is recommended that the antibody be carefully titrated for optimal performance.

Excitation: 488 nm
Emission: 520 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The protein encoded by this gene is a member of the beta chemokine receptor family and predicted to be a seven transmembrane protein similar to G protein-coupled receptors. Chemokines and their receptors regulate thymocyte migration and maturation under normal and inflammatory conditions.

The specific ligand of this receptor is CCL25. It is differentially expressed by T lymphocytes of the small intestine and colon, suggesting a role in thymocyte recruitment and development and functional specialization of immune responses in different segments of the gastrointestinal tract.

This gene is mapped to the chemokine receptor gene cluster region. Two alternatively spliced transcript variants have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지