Thermo Fisher Scientific CD127 Recombinant Mouse Monoclonal Antibody (eBioRDR5), Alexa Fluor Plus 647

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25-0.5 µg/test

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Expression System

ExpiCHO

Class

Recombinant Monoclonal

Type

Antibody

Clone

eBioRDR5

Conjugate

Alexa Fluor™ Plus 647 Alexa Fluor™ Plus 647 Alexa Fluor™ Plus 647

View additional formats

• Unconjugated
• Alexa Fluor Plus 488
• Alexa Fluor Plus 555
• Request custom conjugation

Excitation/Emission Max

658/675 nm View spectra

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein A/G

Storage buffer

proprietary buffer, pH 6.8

Contains

0.008% Bromonitrodioxane, 0.008% Methylisothiazolone

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Wet ice

Product Specific Information

Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.

Applications Tested: This eBioRDR5 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells and HEK293 overexpression cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 658 nm; Emission: 675 nm; Laser: Red Laser

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD127 (Interleukin-7, IL-7) is a glycoprotein involved in the regulation of lymphopoiesis. The response of cells to CD127 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is an alpha/gamma chain heterodimer. CD127 consists of an alpha chain and a gamma chain. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules. CD127 promotes the proliferation of precursor B cells, thymocytes, T cell progenitors, and mature CD4+ and CD8+ T cells. The biological effects of IL7 are mediated by the binding of IL7 to the specific cell surface receptor. Diseases associated with CD127 dysfunction include severe combined immunodeficiency and T cell negative/B cell negative/NK positive severe combined immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.