Thermo Fisher Scientific Ki-67 Monoclonal Antibody (20Raj1), eFluor 660, eBioscience

Thermo Fisher Scientific Ki-67 Monoclonal Antibody (20Raj1), eFluor 660, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 563 / 615 / 737 / 805
• Brilliant Violet 421 / 480 / 605 / 650 / 711 / 786
• eFluor 450 / 506 / 570 / 615
• FITC
• PE / PE-Cyanine5.5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation available

Product Specific Information

The monoclonal antibody 20Raj1 recognizes the human Ki-67 protein. Two isoforms (345 and 395 kDa) are expressed in dividing cells. Ki-67 is detectable during active cell cycle phases (G1, S, G2, M) but absent in resting cells (G0). It localizes to the nucleus during interphase and associates with chromosomes during mitosis, interacting with heterochromatin-bound proteins such as CBX3.

This antibody also recognizes canine Ki-67.
Applications include intracellular staining, microscopy, immunohistochemistry, and immunocytochemistry.

Tested Applications:

• Immunocytochemistry: ≤20 µg/mL
• Immunohistochemistry: ≤20 µg/mL
• Flow Cytometry: ≤0.125 µg/test (100 µL final volume; 10⁵–10⁸ cells/test)

Fluorophore Information:

• eFluor® 660 replaces Alexa Fluor® 647
• Excitation: 633–647 nm
• Emission: 668 nm
• Laser: Red Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

Ki-67 is a nuclear protein expressed during G1, S, G2, and M phases of the cell cycle. It associates with euchromatin and heterochromatin via its FHA domain. Ki-67 is used as a proliferation marker since it is absent in G0 cells and increases through mitosis. It helps determine the growth fraction in neoplasms, correlating with tumor grade and prognosis. Commonly used as a neuronal marker for cell cycling and proliferation.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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