Thermo Fisher Scientific NK1.1 Monoclonal Antibody (PK136), Alexa Fluor 561, eBioscience

Applications

• Flow Cytometry (Flow): Tested up to 0.5 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 660
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 496 / 615 / 661 / 805
• Brilliant Violet 421 / 480 / 605 / 650 / 711 / 786
• eFluor 450
• FITC
• Functional Grade
• PE / PE-Cyanine7 / PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436 / 600 / 645 / 702 / 780
• Request custom conjugation

Product Specific Information

Description:
The PK136 monoclonal antibody reacts with mouse NK1.1, an antigen expressed by natural killer (NK) cells and a subset of T cells in NK1.1-positive strains such as C57BL and NZB. Strains like BALB/c, SJL, AKR, CBA, C3H, and A do not express NK1.1; for these, use monoclonal antibody DX5 (Product #14-5971).

Simultaneous staining of C57BL/6 spleen cells with PK136 and DX5 shows coexpression in most cells, with small DX5⁺PK136⁻ and DX5⁻PK136⁺ populations.

Applications Reported:

• Flow cytometric analysis

Applications Tested:

• Tested on mouse splenocytes
• Recommended ≤ 0.5 µg/test (in 100 µL final volume)
• Optimal titration required per assay
• Alexa Fluor 561 emits at 575 nm, suitable for spectral cytometers
• Can be multiplexed with PE and PE-eFluor 610
• Excitation: 558 nm; Emission: 575 nm; Laser: Yellow-Green

Note:
Internal testing indicates Alexa Fluor 561 may non-specifically stain B cells in Swiss Webster and SJL mice, but not in BALB/c or C57BL/6 strains.

Target Information

NK1.1 (KLRB1 / NKR-P1A / CD161):
NK1.1 identifies natural killer (NK) cells, lymphocytes mediating cytotoxicity and cytokine secretion. The KLRB1 protein, part of the C-type lectin superfamily, includes extracellular, transmembrane, and cytoplasmic domains and functions as a type II membrane protein. Its receptor, NKR-P1A (CD161), recognizes Lectin Like Transcript-1 (LLT1) as a functional ligand.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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