Thermo Fisher Scientific Cdc23 Recombinant Rabbit Monoclonal Antibody (ARC2133)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

ARC2133

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 498-597 of human CDC23 (Q9UJX2) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CDC23,uniProtId:Q9UJX2-1,ncbiNodeId:9606,antigenRange:498-597,antigenLength:597,antigenImageFileName:MA5-38158_CDC23_Q9UJX2-1_Rabbit.svg,antigenImageFileNamePDP:MA5-38158_CDC23_Q9UJX2-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.55 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2898075

Product Specific Information

Positive Samples: HeLa, SKOV3, Jurkat, Mouse lung, Mouse brain, Mouse spleen, Rat brain

Immunogen sequence: YSCGEIVEHL EESTAFRYLA QYYFKCKLWD EASTCAQKCC AFNDTREEGK ALLRQILQLR NQGETPTTEV PAPFFLPASL SANNTPTRRV SPLNLSSVTP

Target Information

Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction. The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s). Ubr2 (Ubiquitin-protein ligase E3-alpha-2), also known as N-recognin-2, is a 1755 amino acid protein that contains one UBR-type zinc finger and one RING-type zinc finger. Participating in protein modification events within the N-end rule pathway, Ubr2 functions as an E3 ubiquitin-protein ligase that recognizes and binds proteins that contain destabilizing N-terminal residues, thereby leading to their ubiquitination and subsequent degradation. Mice lacking Ubr2 are infertile due to defects in male meiosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.