
Thermo Fisher Scientific PYCRL Monoclonal Antibody (OTI5F7), TrueMAB
인간 PYCRL 단백질을 인식하는 마우스 단클론 항체로 WB, ICC/IF, Flow Cytometry에 사용 가능. 동결건조 형태로 제공되며, PBS 버퍼에 trehalose 포함. 연구용으로만 사용되며, 프로린 생합성 관련 효소 연구에 적합.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5F7 |
| Immunogen | Full-length human recombinant PYCRL protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform additional desalting using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
PYCRL encodes a cytoplasmic enzyme belonging to the pyrroline-5-carboxylate reductase family.
This enzyme catalyzes the final step of proline biosynthesis, converting pyrroline-5-carboxylate to proline.
Glutamate and ornithine act as precursors in proline synthesis.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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