Thermo Fisher Scientific AF4 Polyclonal Antibody
Human AF4 단백질을 인식하는 Rabbit Polyclonal Antibody로, Western blot에 최적화되어 있습니다. 항원 친화 크로마토그래피로 정제되었으며, 1 mg/mL 농도의 액상 형태입니다. 4°C에서 보관하며 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
- Western Blot (WB): 1:2,000–1:10,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 1 and 50 of human proto-oncogene AF4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
Target Information
Proto-oncogene AF-4 (FEL) is a product of a chromosomal aberration of the human AFF1 gene, associated with acute leukemias.
The fusion of AF-4 on chromosome band 4q21 with the mixed lineage leukemia (MLL or HRX) gene on 11q23 results in an MLL-AF-4 chimeric transcription factor, where AF-4 contributes transcriptional effector properties and requires cell-specific accessory factors.
MLL is involved in several chromosomal translocations associated with acute myeloid and lymphoid leukemia. The MLL-AF-4 fusion protein is expressed in all normal hematopoietic cells.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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