Thermo Fisher Scientific SIRT5 Monoclonal Antibody (OTI2F1), TrueMAB
SIRT5 단백질을 인식하는 마우스 단클론 항체로, Western blot, IHC, ICC, Flow cytometry에 사용 가능. Human과 Rat 시료에 반응하며, 동결건조 형태로 제공. 연구용으로 SIRT5 관련 단백질 발현 분석에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2F1 |
| Immunogen | Full length human recombinant protein of human SIRT5 produced in HEK293 cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
SIRT5 is a member of the sirtuin (Sir2-like) protein family found in both prokaryotes and eukaryotes.
These proteins share a conserved sirtuin core domain and are involved in NAD metabolism.
Sirtuins may function as mono-ADP-ribosyltransferases, and some reports suggest a link between NAD metabolism and histone deacetylation, indicating a potential reciprocal activation mechanism involving NAD and acetylated histones.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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